黑料社

Time: 10 – 10:30 a.m. EST

Speakers: Jason Koth, Allen Yen

Combinatorial indexing methods for single-cell profiling enable higher levels of sample multiplexing and cell throughput. Using the cell as the compartment, repeated rounds of splitting, indexing, and pooling are performed, enabling single-cell experiments with 100s of thousands to millions of cells.

ScaleBio currently supports workflows for scRNA and scATAC profiling, and is developing kits for single-cell Methylation, Protein, and CRISPR applications. In this presentation, we will discuss our current portfolio, and share relevant examples for neuroscience research.

Time: 11:30 a.m. – noon EST

Speakers: Brice Tiret

Join us on a journey into the realm of integrated neuroscience. With its ever-evolving field, one must position themselves for success with new skills, technologies, and ideas. We are thrilled to present the new nVue and IDEAS platform, designed to enhance productivity and multimodal data potential. Whether you're a scientist, a lab group, or in biotech, our scalable solutions will empower you, saving valuable time and effort. Don't miss this opportunity to transform your neuroscience endeavors!

Time: 1 – 1:30 p.m. EST

Speakers: Hwai-Jong Cheng; Jung-Chi Liao

Syncell is introducing Microscoop, a platform combining microscopy imaging, AI-enabled pattern generation, and pattern illumination-induced photo-biotinylation to enable subcellular spatial proteomic discovery. It has been successfully used to identify novel protein players in beta amyloid, ALS pTDP-43 aggregates, mossy fiber boutons, etc. As a subcellular pickable microscope, Syncell Microscoop can be widely useful for hypothesis-free subcellular proteomic discovery beyond mapping.

Time: 2:30 – 3 p.m. EST

Speakers: George Portugal

Modern neuroscience research benefits from holistic investigation approaches by integrating technologies to study neurological processes at molecular, cellular, and animal levels. Harvard Bioscience (HBIO) is a global leader in developing solutions to support neuroscience research and drug discovery. HBIO aims to mitigate laboratory challenges by assisting neuroscientists in a broad spectrum of research needs, furnishing them with diverse, integrated tools that range from in vitro to in vivo.

Time: 4 – 4:30 p.m. EST

Speakers: Caryn Carson

Western blotting is a key technique in neuroscience. It is used to determine relative protein levels, often as proof of differences between cell types, changes under a disease state, or successful genetic knockouts. In this seminar, we will discuss important factors and methods to generate publication-quality Western blot data, such as best practices in sample preparation, controls and replicates, how to choose your antibodies, imaging, and data analysis and normalization.

Time: 10 – 10:30 a.m. EST

Speakers: Marisa Biondi, Nicole Celin

The Tobii Team will take you from the conceptualization and design of an eye tracking study, through live data collection with the Tobii Pro Glasses 3, to end by showcasing the qualitative and quantitative analysis features in Tobii Pro Lab. You’ll leave with the knowledge of how to get your research from the laboratory out into any kind of testing environment, with real-world examples. Attendees will have an exclusive opportunity to enter a raffle to win a one-month free trial of either Tobii Pro Glasses 3 or a Tobii Pro Fusion!

Time: 11:30 a.m. – noon EST

Speakers: Vik Devgan, Adrian Oblak

NanoString’s integrated, multiomic solutions allows the study of neurodegeneration, neuroinflammation, and neuronal development from spatial single cells to multicellular tissue compartments and patient cohorts. Discover and develop prognostic and spatial gene signatures. Characterize the heterogeneity of brain tissue in health and disease. Reveal functional, cellular, and temporal changes spatially at single-cell and subcellular resolution. Hear Dr. Adrian Oblak’s spatial Alzheimer's study.

Time: 1 – 1:30 p.m. EST

Speakers: Joseph Beechem, Wei Tian

Make the leap into spatial, single cell imaging with CosMx SMI, with best-in-class cell segmentation, cell atlasing, and biomarker discovery. Learn about the future of CosMx SMI with same slide, highest-plex spatial proteogenomics using 6K RNA + 64-plex protein human neuro assays. For mouse, a comprehensive pairing of 1K RNA+ 64-plex protein for neuropathology. Hear from Joe Ecker’s lab about highest-plex single cell spatial signaling and mapping on human brain, diseased, and normal tissues.

Time: 2:30 – 3 p.m. EST

Speakers: Jimmy Fong, Kevin Mann

Two-photon (2P) imaging enables the visualization of neural dynamics across vast areas and at faster rates across cortical layers. Combined with spatial light modulators like the Bruker Neuralight 3D Ultra and fast volumetric scanning with our electrotunable lens, our 2P systems optogenetically stimulate/monitor 3D functional connectivity in real-time. Now with NeuraLeap for Ultima 2Pplus, learn about a new form of activity mapping with super-fast focus capturing correlation across brain layers.

Time: 4 – 4:30 p.m. EST

Speakers: Elisa De Launoit

Imaging 3D samples with light sheet microscopy demands significant time investments. However, two releases for the UltraMicroscope Blaze™ have significantly streamlined sample acquisitions. The new LightSpeed mode enhances imaging speeds by 50 times and when coupled with the latest software innovation, MACS® iQ View, the entire imaging process becomes user-friendly. We will illustrate how we leveraged these upgrades to study the intricacies of vascular networks within the developing brain.

Time: 10 – 10:30 a.m. EST

Speakers: Molly Heyer, Marie Lee

Explore more of your regions of interest with the Curio Seeker 10 x 10 mm tile, a spatial transcriptomics technology that provides single-cell resolution while preserving tissue architecture and cellular neighborhoods of fresh frozen tissue samples. We will discuss the latest data from whole, adult murine brain, and demonstrate how the Curio Seeker 10 x 10 mm tile can delineate different cell populations in distinct regions of the cerebral cortex.

Time: 11:30 a.m. – noon EST

Speakers: Winfried Wiegraebe

Single-Molecule Localization Microscopy (SMLM) is a powerful tool for neuroscience research, allowing for the study of synapses and neurons at a high resolution of 20 nm. The Bruker Vutara VXL super-resolution microscope offers several advantages, including 3D imaging, multi-camera imaging, integrated fluidics and multiplexing, and easy-to-use software. This presentation will discuss the basics of super-resolution microscopy and how the Vutara VXL can advance neuroscience research. Join us to learn more!

Time: 1 – 1:30 p.m. EST

Speakers: George Glekas

Starting in 1919, Olympus (now Evident) began to revolutionize imaging technology. Over our 100-year history as a global leader in imaging technologies and cutting-edge design, we have reimagined the traditional microscope for various applications. Recently we have developed new manufacturing technology that will enable higher-quality imaging with our X Line objectives. Join us as we unveil our next generation of precision imaging technology that will broaden the spectrum of possibilities for your research.

Time: 2:30 – 3 p.m. EST

Speakers: Allysa Stern

The workflows associated with developing iPSCs into 3D neuronal organoids are time-consuming, manual, and suffer from limitations of heterogeneity. To achieve a scalable and reliable method for growing iPSC-derived neuronal organoids, we used CellRaft Technology, which provides an automated, user-friendly, high-throughput solution for accelerating the testing of iPSC-derived neuronal organoids in toxicity, disease modeling, and drug discovery.

Time: 4 – 4:30 p.m. EST

Speakers: Javier Conde Vancells, Malika Bsibsi

For years, widely used protocols for generating hiPSC-derived microglia have required long, complex workflows that suffer from lot-to-lot consistency. Dr Javier Conde Vancells, Director Product Management, bit.bio and Dr Malika Bsibsi, Research Leader, Charles River Laboratories, will share exclusive data on the functional and consistent properties of hiPSC-derived ioMicroglia. Highlighting their use in assays, including phagocytosis, cytokine secretion and chemotaxis in just 10 days post-thaw.

Time: 11:30 a.m. – noon EST

Speakers: Edwin Pratt; Tyler Lalonde

Explore the realm of PET imaging and the new concept of multiplexed PET. This scientific advancement makes it possible to perform simultaneous imaging with 2 different isotopes providing more in-depth information with a single scan.

Multiplexed PET uses prompt gamma emissions from positron-gamma emitters to enable truly simultaneous dual-isotope PET imaging. This reconstruction method allows PET scanners to record events beyond standard coincidences and can be used for dual PET tracer studies.